- Zebrafish lines
- Zebrafish Facilities
- Anti-angiogenesis assays
- Acute Toxicity Assays
- Developmental Toxicity Assays
- Drug screening and discovery
- Producing genetically modified fish
- Using disease models and high throughput phenotyping screening
How can assess acute toxicity with the cost/time-effective screening?
Introduction
Royan Zebrafish Core Facility is a research laboratory offering the newest and most up-to-date research that can accompany your studies. Zebrafish (Danio rerio) are introduced by small size, enormous offspring, and phenotype similarities with human as well as the most time/cost-effective screening that helps to recognize acute toxicities.
For fast and cost/time-effective screening, the best offer will be Guideline 236 approved by the FDA which assesses the acute toxicity of chemicals and compounds utilized in drug discovery, environmental risk, and poisons.
Method Description
Embryos with 5 different concentrations of the compound are incubated from 0 to 96 hours post-fertilization (hpf).
Readouts
To evaluate toxicity, fertilized eggs are exposed to chemicals and compounds for 96 hours. Every 24 hours, lethality is recorded by 4 indicators observations including: 1) coagulation of fertilized eggs 2) lack of somite formation 3) lack of detachment of the tailbud from the yolk sac, and 4) lack of heartbeat. Based on these indicators LD50 is calculated at 96 hpf and the concentration-effect curve determines the following parameters consisting of 1) no observed effect concentration, 2) lowest observed effect concentration, 3) 100 % lethal concentration, and 4) lethal concentration 50 (LC50).
Figures
Figure 1: the endpoint of the zebrafish acute toxicity